王显花

Email:xianhua@pku.edu.cn

所在院系:未来技术学院

主页:http://scholar.pku.edu.cn/xianhuawang/publications?page=1

1996-2000年就读于北京大学生命科学学院生物化学与分子生物学专业,获理学学士学位;2000-2005年于北京大学生命科学学院硕博连读,获理学博士学位;2005-2007年在北京大学分子医学研究所钙信号转导实验室进行博士后期间的研究;2009-2010年在美国健康卫生研究院(NIH)老年研究所进行合作研究;2007-今就职于北京大学分子研究研究所。2010年获得美国健康卫生研究院FARE奖。

主要研究兴趣:1)线粒体ROS信号,尤其是量子化“超氧炫”信号的生理功能和产生机制;2)线粒体膜通透性孔道(mPTP)分子组份的鉴定。

教育背景

1996-2000年:北京大学生命科学学院,生物化学与分子生物学专业,获理学学士学位;

2000-2005年:北京大学生命科学学院硕博连读,获理学博士学位;

工作经历

2005-2007年:北京大学分子医学研究所钙信号转导实验室进行博士后期间的研究;

2009-2010年:在美国健康卫生研究院(NIH)老年研究所进行合作研究;

2007-今:就职于北京大学分子研究研究所。

科研成果

2014

Jian C, Hou T, Yin RK, Cheng H, Wang X. Regulation of superoxide flashes by transient and steady mitochondrial calcium elevations. Science China Life Sciences. 2014;(5):495-501.

Shang W, Lu F, Sun T, Xu J, Li L-L, Wang Y, Wang G, Chen L, Wang X, Cannell MB, et al. Imaging Ca2+ nanosparks in heart with a new targeted biosensor. Circulation Research [Internet]. 2014;(3):412-420. 访问链接

Shen E-Z, Song C-Q, Lin Y, Zhang W-H, Su P-F, Liu W-Y, Zhang P, Xu J, Lin N, Zhan C, et al. Mitoflash frequency in early adulthood predicts lifespan in caenorhabditis elegans. Nature [Internet]. 2014;(1):128-132. 访问链接

2013

Cao Y, Zhang X, Shang W, Xu J, Wang X, Hu X, Ao Y, Cheng H. Proinflammatory Cytokines Stimulate Mitochondrial Superoxide Flashes in Articular Chondrocytes In Vitro and In Situ. PLoS ONE. 2013;(6).

Wang J-Q, Chen Q, Wang X, Wang Q-C, Wang Y, Cheng H-P, Guo C, Sun Q, Chen Q, Tang T-S. Dysregulation of mitochondrial calcium signaling and superoxide flashes cause mitochondrial genomic DNA damage in Huntington disease. Journal of Biological Chemistry [Internet]. 2013;(5):3070-3084. 访问链接

Wang X, Fang H, Huang Z, Shang W, Hou T, Cheng A, Cheng H. Imaging ROS signaling in cells and animals. Journal of Molecular Medicine [Internet]. 2013;(8):917-927. 访问链接

Huang X, Sun L, Ji S, Zhao T, Zhang W, Xu J, Zhang J, Wang Y, Wang X, Franzini-Armstrong C, et al. Kissing and nanotunneling mediate intermitochondrial communication in the heart. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2013;(8):2846-2851. 访问链接

Lan W-LP, Gong G, Gerstner BJ, Ducreux S, Yule DI, Pouvreau S, Wang .X, Sheu S-S, Cheng H, Dirksen RT, et al. Respective contribution of mitochondrial Superoxide and ph to mitochondria-targeted circularly permuted yellow fluorescent protein (mt-cpYFP) flash activity. Journal of Biological Chemistry [Internet]. 2013;(15):10567-10577. 访问链接

Zhang H, Gomez AM, Wang X, Yan Y, Zheng M, Cheng H. ROS regulation of microdomain Ca2+ signalling at the dyads. Cardiovascular Research [Internet]. 2013;(2):248-258. 访问链接

Zhang X, Huang Z, Hou T, Xu J, Wang Y, Shang W, Ye T, Cheng H, Gao F, Wang X. Superoxide constitutes a major signal of mitochondrial superoxide flash. Life Sciences [Internet]. 2013;(4):178-186. 访问链接

Hou T, Zhang X, Xu J, Jian C, Huang Z, Ye T, Hu K, Zheng M, Gao F, Wang X, et al. Synergistic triggering of superoxide flashes by mitochondrial Ca 2+ uniport and basal reactive oxygen species elevation. Journal of Biological Chemistry [Internet]. 2013;(7):4602-4612. 访问链接

Zhang H, Shang W, Zhang X, Gu J, Wang X, Zheng M, Wang Y, Zhou Z, Cao J-M, Ji G, et al. β-Adrenergic-stimulated l-type channel Ca2+ entry mediates hypoxic Ca2+ overload in intact heart. Journal of Molecular and Cellular Cardiology [Internet]. 2013:51-58. 访问链接

2012

Wei C, Wang X, Zheng M, Cheng H. Calcium gradients underlying cell migration. Current Opinion in Cell Biology [Internet]. 2012;(2):254-261. 访问链接

Zhao T, Huang X, Han L, Wang X, Cheng H, Zhao Y, Chen Q, Chen J, Cheng H, Xiao R, et al. Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes. Journal of Biological Chemistry [Internet]. 2012;(28):23615-23625. 访问链接

Lin P, Zhu H, Cai C, Wang X, Cao C, Xiao R, Pan Z, Weisleder N, Takeshima H, Jianjie M. Nonmuscle myosin IIA facilitates vesicle trafficking for MG53-mediated cell membrane repair. FASEB Journal [Internet]. 2012;(5):1875-1883. 访问链接

Weisleder N, Takizawa N, Lin P, Wang X, Cao C, Zhang Y, Tan T, Ferrante C, Zhu H, Chen P-J, et al. Recombinant MG53 protein modulates therapeutic cell membrane repair in treatment of muscular dystrophy. Science Translational Medicine [Internet]. 2012;(139). 访问链接

Wang X, Jian C, Zhang X, Huang Z, Xu J, Hou T, Shang W, Ding Y, Zhang W, Ouyang M, et al. Superoxide flashes: Elemental events of mitochondrial ROS signaling in the heart. Journal of Molecular and Cellular Cardiology [Internet]. 2012;(5):940-948. 访问链接

Li K, Zhang W, Fang H, Xie W, Liu J, Zheng M, Wang X, Wang W, Tan W, Cheng H. Superoxide flashes reveal novel properties of mitochondrial reactive oxygen species excitability in cardiomyocytes. Biophysical Journal [Internet]. 2012;(5):1011-1021. 访问链接

2011

Chen M, Wang Y, Hou T, Zhang H, Qu A, Wang X. Differential mitochondrial calcium responses in different cell types detected with a mitochondrial calcium fluorescent indicator, mito-GCaMP2. Acta Biochimica et Biophysica Sinica [Internet]. 2011;(10):822-830. 访问链接

Fang H, Chen M, Ding Y, Shang W, Xu J, Zhang X, Zhang W, Li K, Xiao Y, Gao F, et al. Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals. Cell Research [Internet]. 2011;(9):1295-1304. 访问链接

Huang Z, Zhang W, Gong G, Fang H, Zheng M, Wang X, Xu J, Dirksen RT, Sheu S-S, Cheng H, et al. Response to "a critical evaluation of cpYFP as a probe for superoxide". Free Radical Biology and Medicine [Internet]. 2011;(10):1937-1940. 访问链接

Ma Q, Fang H, Shang W, Liu L, Xu Z, Ye T, Wang X, Zheng M, Chen Q, Cheng H. Superoxide flashes: Early mitochondrial signals for oxidative stress-induced apoptosis. Journal of Biological Chemistry [Internet]. 2011;(31):27573-27581. 访问链接

Chakir K, Zhu W, Tsang S, Woo AY-H, Yang D, Wang X, Zeng X, Rhee M-H, Mende U, Koitabashi N, et al. RGS2 is a primary terminator of β2-adrenergic receptor-mediated Gi signaling. Journal of Molecular and Cellular Cardiology [Internet]. 2011;(6):1000-1007. 访问链接

2010

Wang X, Xie W, Zhang Y, Lin P, Han L, Han P, Wang Y, Chen Z, Ji G, Zheng M, et al. Cardioprotection of Ischemia/Reperfusion injury by cholesterol-dependent MG53-mediated membrane repair. Circulation Research [Internet]. 2010;(1):76-83. 访问链接

Xie W, Brochet DXP, Wei S, Wang X, Cheng H. Deciphering ryanodine receptor array operation in cardiac myocytes. Journal of General Physiology [Internet]. 2010;(2):129-133. 访问链接

Wei C, Wang X, Chen M, Ouyang K, Zheng M, Cheng H. Flickering calcium microdomains signal turning of migrating cells. Canadian Journal of Physiology and Pharmacology [Internet]. 2010;(2):105-110. 访问链接

Cao C-M, Zhang Y, Weisleder N, Ferrante C, Wang X, Lv F, Zhang Y, Song R, Hwang M, Jin L, et al. MG53 constitutes a primary determinant of cardiac ischemic preconditioning. Circulation [Internet]. 2010;(23):2565-2574. 访问链接

2009

Wei C, Wang X, Chen M, Ouyang K, Song L-S, Cheng H. Calcium flickers steer cell migration. Nature [Internet]. 2009;(7231):901-905. 访问链接

2008

Wang W, Fang H, Groom L, Cheng A, Zhang W, Liu J, Wang X, Li K, Han P, Zheng M, et al. Superoxide Flashes in Single Mitochondria. Cell [Internet]. 2008;(2):279-290. 访问链接

2007

Xiao B, Tian X, Xie W, Jones PP, Cai S, Wang X, Jiang D, Kong H, Zhang L, Chen K, et al. Functional consequence of protein kinase A-dependent phosphorylation of the cardiac ryanodine receptor: Sensitization of store overload-induced Ca 2+ release. Journal of Biological Chemistry [Internet]. 2007;(41):30256-30264. 访问链接

2006

Yao L-J, Wang G, Ou-Yang K-F, Wei C-L, Wang X-H, Wang S-R, Yao W, Huang H-P, Luo J-H, Wu C-H, et al. Ca2+ sparks and Ca2+ glows in superior cervical ganglion neurons. Acta Pharmacologica Sinica [Internet]. 2006;(7):848-852. 访问链接

2005

Ouyang K, Zheng H, Qin X, Zhang C, Yang D, Wang X, Wu C, Zhou Z, Cheng H. Ca2+ sparks and secretion in dorsal root ganglion neurons. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2005;(34):12259-12264. 访问链接