研究方向

化学与生命科学的交叉融合为分析、理解和操控生物学过程开辟了一条全新的研究途径。我们课题组的研究兴趣集中于化学糖生物学。我们开发化学方法，用以研究糖基化的生物学功能及其在相关人类疾病中的作用。主要研究内容包括：

1. 活细胞和活体中聚糖的化学标记、成像及组学分析

2. 脑部糖基化的生理及病理功能

3. 糖基化在蛋白质-RNA相互作用中的功能

4. 生物大分子（糖、蛋白、核酸）的代谢标记方法开发

代表性科研论文

1. Gao, L.; Song, Q.; Liang, H.; Zhu, Y.; Wei, T.; Dong, N.; Xiao, J.; Shao, F.;Chen, X.*“LegionellaEffector SetA as A General O-glucosyltransferase for Eukaryotic Proteins”Nat. Chem. Biol.15, 213-216 (2019).

2. Huang, R.; Han, M.; Meng, L.;Chen, X.* “Transcriptome-Wide Discovery of Coding and Noncoding RNA-Binding Proteins”Proc. Natl. Acad. Sci. USA115, E3879-E3887 (2018).

3. Sun, Y.; Hong, S.; Xie, R.; Huang, R.; Lei, R.; Cheng, B.; Sun, D.; Du, Y.; Nycholat, C.; Paulson, J.;Chen, X.* “Mechanistic Investigation and Multiplexing of Liposome-Assisted Metabolic Glycan Labeling”J. Am. Chem. Soc.140, 3592-3602 (2018).

4. Qin, W.; Qin, K.; Fan, X.; Peng, L.; Hong, W.; Zhu, Y.; Lv, P.; Du, Y.; Huang, R.; Han, M.; Cheng, B.; Liu, Y.; Zhou, W.; Wang, C.*;Chen, X.* “Artifical Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling”Angew. Chem. Int. Ed.57, 1817-1820 (2018).

5. Qin, W.; Lv, P.; Fan, X.; Quan, B.; Zhu, Y.; Qin, K.; Chen, Y.; Wang, C.*;Chen, X.* “Quantitative Time-Resolved Chemoproteomics Reveals Stable O-GlcNAc Regulates Box C/D snoRNP Biogenesis”Proc. Natl. Acad. Sci. USA114, E6749-E6758 (2017).

6. Zhu, Y.; Wu, J.;Chen, X.* “Metabolic Labeling and Imaging of N-Linked Glycans inArabidopsis Thaliana”Angew. Chem. Int. Ed 55, 9301-9305 (2016).

7. Xie, R.; Dong, L.; Du, Y.; Zhu, Y.; Hua, R.; Zhang, C.;Chen, X.* “In VivoMetabolic Labeling of Sialoglycans in the Mouse Brain By Using A Liposome-Assisted Bioorthogonal Reporter Strategy”Proc. Natl. Acad. Sci. USA113, 5173-5178(2016).

8. Lin, L.; Liu, L.; Zhao, B.; Xie, R.; Lin, W.; Li, H.; Li, Y.; Shi, M.; Chen, Y.; Springer, T. A.;Chen, X.* “Carbon Nanotube-Assisted Optical Activation of TGF- Signaling by Near-Infrared Light”Nat. Nanotech.10, 465-471 (2015).