研究方向

细胞增殖时DNA代表的遗传信息被忠实复制分配到子代细胞，同时染色质结构承载的表观遗传信息通过DNA复制偶联的核小体组装系统将组蛋白分配给子代DNA。此过程调节生命个体的正常发育，其紊乱与癌症、神经退行性病变等人类疾病密切相关。本实验室以酵母和哺乳动物细胞为模式，从DNA复制偶联的核小体组装入手，致力于探索染色质复制与表观遗传信息传递的分子机制，围绕解析染色质复制的机制，研究染色质复制在表观遗传信息传递中的作用，探讨表观遗传信息，基因组稳定性和细胞命运决定之间的关系。

研究方向主要包括：

1、DNA复制偶联的核小体组装机制；

2、表观遗传信息在子代细胞的分配机制；

3、染色质复制维持基因组稳定性的机制。

代表性论文

1. Li, S.Q^#., Dong, Z.Q^#., Yang, S.S., Feng, J.X. andLi, Q*(2019)Chaperoning RPA during DNA metabolism.Current Geneticsdoi: 10.1007/s00294-019-00945-3. Review

2. Li, Q*., Zhang, X., and Zhang, Z.* (2018). CHAF1B overexpression: A brake for the differentiation of leukemia cells.Cancer Cell34:693-694. Preview

3. Li, S.Q^#, Xu, Z.Y.^#, Xu, J.W.^#, Zuo, L.Y., Yu, C.H., Zheng, P., Gan, H.Y., Wang, X.Z., Li, L.T., Sharma, S., Chabes, A., Li, D., Wang, S., Zheng, S.H., Li, J.B., Chen, X.F., Sun, Y.J., Xu, D.Y., Han, J.H., Chan, K.M., Qi, Z., Feng, J.X.*, andLi, Q.* (2018) Rtt105 functions as a chaperone for replication protein A to preserve genome stabilityThe EMBO Journale99154.

4. Liu, S.F.^#, Xu, Z.Y.^#, Leng, H.^#, Zheng, P., Yang, J.Y., Chen, K.F., Feng, J.X.,Li, Q.* (2017).RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly.Science355, 415-420.

5. Feng, J.X.^＃, Gan, H.Y.^＃, Eaton, M.L., Zhou, H., Li, S.Q., Belsky, J.A., MacAlpine, D.M., Zhang, Z.G.* andLi, Q.* (2016). Noncoding transcription is a driving force for nucleosome instability inspt16mutant cells.Molecular and Cellular Biology36,1856-1867.Article of significant interest selected as a Spotlight.

6. Yang, J.Y.^＃, Zhang, X.^＃, Feng, J.X.^＃, Leng, H., Li, S.Q., Xiao, J.X., Liu, S.F., Xu, Z.Y., Xu, J.W., Li, D., Wang, Z.S., Wang, J.Y., andLi, Q.* (2016). The histone chaperone FACT contributes to DNA replication-coupled nucleosome assembly.Cell Reports14,1128-1141.

7. Su, D.^#, Hu, Q.^#,Li, Q.^#,Thompson, J.R., Cui, G.F., Fazly, A., Davies, B.A., Botuyan, M.V., Zhang, Z.G.* and Mer, G.* (2012). Structural basis for recognition of H3K56-acetylated histone H3–H4 by the chaperone Rtt106.Nature483,104-107.

8. Li, Q.^＃,Zhou, H.^＃, Wurtele, H., Davies, B., Horazdovasky, B., Verreault, A.*and Zhang, Z.G.*(2008). Acetylation of Histone H3 lysine 56 regulates replication-coupled nucleosome assembly.Cell134,244-255.Previewed in the same issue. Highlighted inNature Reviews Molecular Cell Biology, September 2008