研究方向

高宁实验室主要致力于阐明细胞内大型蛋白-核酸复合物形成的分子机器的精细结构及工作机制，近年来的科研工作着重于核糖体的生物生成（ribosome biogenesis）、蛋白质生物合成的调控、DNA复制起始调控等重要基础生物学过程。实验室主要采用冷冻电镜三维重构的方法分析大型复合物的高分辨结构，辅助遗传学、细胞生物学、生化分子生物学手段回答大分子机器在功能执行过程中的机制性问题。同时，针对结构课题中的技术难点，实验室还致力于冷冻电镜方法学的研究，包括样品制备技术和算法的改进等。

代表性论文

1. Jiang, F., Li, N., Wang, X., Cheng, J., Huang, Y., Yang, Y., Yang,J., Cai, B., Wang, Y.P., Jin, Q.#, andGao, N.#(2019). Cryo-EM Structure and Assembly of anExtracellular Contractile Injection System.Cell177, 370-383e315.

2. Li, N., Lam, W.H., Zhai, Y.#, Cheng, J., Cheng, E., Zhao, Y.,Gao, N.#,and Tye, B.K.#(2018). Structure of the origin recognition complex bound to DNA replicationorigin.Nature559, 217-222.

3. Ma, C., Kurita, D., Li, N., Chen, Y., Himeno, H.#, andGao, N.#(2017).Mechanistic insights into the alternative translation termination by ArfAand RF2.Nature541, 550-553.

4. Ma, C., Wu, S., Li, N., Chen, Y., Yan, K., Li, Z., Zheng, L., Lei,J., Woolford, J.L., Jr.#, andGao,N.#(2017). Structural snapshot of cytoplasmic pre-60S ribosomalparticles bound by Nmd3, Lsg1, Tif6 and Reh1.Nat Struct Mol Biol24, 214-220.

5. Wu, S., Tutuncuoglu, B., Yan, K., Brown, H., Zhang, Y., Tan, D.,Gamalinda, M., Yuan, Y., Li, Z., Jakovljevic, J., Ma, C., Lei, J., Dong,M.-Q., Woolford, J.L.#, andGao,N.#(2016). Diverse roles of assembly factors revealed bystructures of late nuclear pre-60S ribosomes.Nature534, 133-137.

6. Gu, J., Wu, M., Guo, R., Yan, K., Lei, J.,Gao, N.#, and Yang, M.# (2016) The architecture ofmammalian respirasome,Nature, 537, 639-643.

7. Li, N., Zhai, Y.#, Zhang, Y., Li, W., Yang, M., Lei, J., Tye, B.K.#,andGao, N#. (2015).Structure of the eukaryotic MCM complex at 3.8 A.Nature524, 186-191.

8. Zhang, Y., Mandava, C.S., Cao, W., Li, X., Zhang, D., Li, N., Zhang,Y., Zhang, X., Qin, Y., Mi, K., Lei, J.#, Sanyal, S.#, andGao, N.# (2015). HflX is aribosome-splitting factor rescuing stalled ribosomes under stressconditions.Nat Struct Mol Biol22, 906-913.